Isolation and Identification of a Sexual Hormone in Yeast |
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| Authors: | Akira Sakurai Saburo Tamura Naohiko Yanagishima Chikashi Shimoda Michio Hagiya Narao Takao |
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| Institution: | 1. The Institute of Physical and Chemical Research, Wako-shi, Saitama-ken;2. Department of Biology, Osaka City University, Osaka;3. Kobe Women’s College of Pharmacy, Kobe |
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| Abstract: | 5-Fluorotryptophan (5FT), indolmycin (IM), 4-fluorotryptophan and 7-azatryptophan were found on screening to be tryptophan antagonists among various chemically synthesized and naturally occurring tryptophan analogues for the isolation of l-tryptophan (l-Trp) producing mutants of Bacillus subtilis K.From among 5FT resistant mutants, potent l-Trp producers were obtained using an improved isolation medium. Growth of the isolated 5FT-resistant l-Trp producer, AJ 11709, was inhibited by IM. From among 5FT and IM resistant mutants, the best strain, AJ 11979, which produced 9.0 g/liter of l-Trp from 13% glucose on 120hr cultivation, was selected. |
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| Keywords: | eicosatrienoic acid lipid metabolism non-methylene-interrupted polyunsaturated fatty acid sciadonic acid Torreya nucifera |
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