Neutral Proteinases I and II of Aspergillus sojae |
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Authors: | Hiroshi Sekine |
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Institution: | Noda Institute for Scientific Research Noda-shi, Chiba-ken, Japan |
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Abstract: | Epimerization of (?)-isodihydrocarvone (I) to (?)-dihydrocarvone (II) by Pseudomonas fragi IFO 3458 was studied. I was easily epimerized to II by the growing cells, the resting cells or the cell-free extracts.An epimerase catalyzing the conversion of I to II was partially purified from the bacterial extracts about 56-fold with heat-treatment, ammonium sulfate precipitation and DEAE-Sephadex A-50 column chromatography. By the action of this epimerase, the ratio of I to II becomes about 25: 75 (K=3). It appeared that the epimerase is very stable to heat; the activity of epimerization remains 66 and 36% after treatment at 97°C for 60 and 120 min, respectively. |
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Keywords: | Vibrio anguillarum outer membrane vesicle pathogen cytokine |
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