Biosynthesis of Leaf Alcohol: Kinetic Study of Lipoxygenase Activation Process Caused by Hydroperoxylinoleic Acid |
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Authors: | Hitoshi Aoshima Tadahiko Kajiwara Akikazu Hatanaka Hiroshi Nakatani Keitaro Hiromi |
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Institution: | 1. Department of Chemistry;2. Department of Agricultural Chemistry, Yamaguchi University, Yamaguchi 753;3. Department of Food Science and Technology, Kyoto University, Kyoto 606 |
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Abstract: | Quaternary structure of ribulose-1, 5-bisphosphate (RuP2) carboxylase from the autotrophically grown cells of blue-green alga, Anabaena cylindrica, was studied. Sedimentation coefficient (s20, w) of the enzyme was determined to be 18.3 S by the sucrose density gradient centrifugation. The molecular weight was estimated to be 5.0 × 105 by the Sepharose 4B gel filtration technique. The purification of the enzyme from the algal cells was undertaken by means of sucrose density gradient centrifugation and DEAE-Sephadex A–50 ion-exchange column chromatography, and the structural make-up of the enzyme containing two subunits, A (M. W., 5.2 × 104) and B (M. W., 1.2 × 104) was established by the Na-dodecylsulfate polyacrylamide gel electrophoresis experiment. Structural similarity of the algal RuP2carboxylase with the spinach enzyme was further demonstrated by the Ouchterlony double immunodiffusion experiment. |
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Keywords: | ADR1 alcoholic fermentation glucose derepression Saccharomyces cerevisiae sake yeast |
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