Isolation of a New Root Growth-stimulating Substance from a Fungus |
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Authors: | Takeshi Sassa Kazuo Tomizuka Michimasa Ikeda Yukichi Miura |
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Institution: | Department of Agricultural Chemistry, Yamagata University, Tsuruoka, Yamagata, Japan |
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Abstract: | In the last few decades, enzymatic production of 3,4-dihydroxyphenyl-L-alanine (L-dopa) using tyrosine phenol-lyase (Tpl) has been industrialized. This method has an intrinsic problem of tyrosine contamination because Tpl is synthesized under tyrosine-induced conditions. Herein, we constructed a hyper-L-dopa-producing strain by exploiting a mutant TyrR, an activator of tpl. The highest productivity was obtained for the strain grown under non-induced conditions. It was 30-fold higher than that obtained for tyrosine-induced wild-type cells. |
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