Milk-clotting Enzyme from Microorganisms

The milk-clotting activity of Mucor-rennin (Milk clotting enzyme of Mucor pusillus Lindt) was inhibited by reaction of diazo-l-H-tetrazole accompanied with increase of the value of the absorbance of biazo-histidine at 480 nm. The activity did not remain when the absorbance reached 50% of maximum value. It is concluded from these results that one mole of histidine in 2 moles of histidine contained in the enzyme has a relation to active center.