Separation and Identification of UDP-N-Acetylhexosamine Derivatives Excreted by Penicillin-treated Corynebacterium alkanolyticum |
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| Authors: | Tsuneo Kanamaru Masakazu Kikuchi Yoshio Nakao |
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| Institution: | Microbiological Research Laboratories, Central Research Division, Takeda Chemical Industries, Ltd., Osaka, Japan |
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| Abstract: | N-Acetylhexosamine derivatives, which are intermediates of cell wall synthesis, were detected in UV-absorbing substances excreted by penicillin-treated Corynebacterium alkanolyticum. Gel filtration, using Sephadex G-25, separated N-acetylhexosamine derivatives to three components, each of which was purified by Dowex 1 × 2 column and paper chromatographies.From the analytical studies, N-acetylhexosamine derivatives were found to be composed of UDP-N-acetylmuramic acid-(diaminopimelic acid, glutamic acid, alanine), UDP-N-acetylhexosaminuronic acid and UDP-N-acetylglucosamine. |
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