Participation of RNase I in MS2 Phage Growth |
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| Authors: | Kanji Ohyama Tohru Komano Konoshin Onodera |
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| Institution: | Laboratory of Biochemistry, Department of Agricultural Chemistry, Kyoto University, Kyoto |
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| Abstract: | Participation of RNase I in the growth of phage on infection with bacteriophage MS2 was studied.Some strains of uracil-requiring E. coli were isolated, grown in MS broth, and transferred to a minimal medium to exhaust the pool of nucleotides. The phage was then added to the cells grown in uracil-deficient medium. The growth of phage was observed to occur at the burst size of two hundreds in strains of E. coli K12S (F+) U? and C600 (F+) U?, which possessed RNase I, but not in strains, A19 (Hfr) U? and Q13 (Hfr) U?, which lacked RNase I.A marked increase in acid-soluble fraction was observed with E. coli K12S (F+) U? and C600 (F+) U?, whereas the increase was little with E. coli A10 (Hfr) U? and Q13 (Hfr) U? Conditions for the growth of phage in uracil-deficient medium were investigated and the effect of antibiotics were also investigated. |
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