Production of Nucleic Acid-Related Substances by Fermentative Processes |
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Authors: | Haruo Tanaka Zenroku Sato Kiyoshi Nakayama Shukuo Kinoshita |
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Affiliation: | Tokyo Research Laboratory, Kyowa Hakko Kogyo Co., Ltd., Machida, Tokyo |
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Abstract: | During the course of the investigation on the production of nucleotide by fermentative processes, it was found that a large amount of ATP and ADP or GTP and GDP, in addition to a smaller amount of AMP or GMP, accumulated in the culture broth when Brevibacterium ammoniagenes ATCC 6872 was incubated in a medium containing adenine or guanine.After treatment of the culture filtrate with charcoal, the nucleotides were isolated by ion-exchange chromatography on Dowex-1 × 2 (Cl?-form). They were identified by paper-chromatography, ultraviolet absorption spectra and analyses of base, ribose and phosphate. The ATP preparation from the broth had the same activity with that of authentic sample in the β-aspartokinase system from Corynebacterium glutamicum. |
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