Identification of l-Dopa and Protocatechuic Acid as Main Precursor of Pigment of Brown Rice-koji |
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Authors: | Toshiteru Ohba Hideya Murakami Shodo Hara |
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Affiliation: | Research Institute of Brewing, 2-6 Takinogawa, Kita-ku, Tokyo |
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Abstract: | The rice koji for sake making usually shows white, but sometimes, during storage in the air it turns brown. This turn is accelerated by some wet conditions.Three kinds of precursor of the pigment in browned rice-koji were isolated in the present work by column chromatography. A main precursor was adsorbed on alumina at pH 8.5 and eluted with 0.3 n acetic acid. The eluate was then treated by Dowex 50-X8 (H) column, eluted with water and 1 n HCl, and then identified as l-dopa after derived to 3,4-di-O-benzoyl-N-benzoyl-l-dopa ethyl ester. The second precursor was isolated from ethylether soluble fraction of the water eluate described above and identified as protocatechuic acid by some chemical properties. The third precursor was not isolated owing to its chemical unstableness, though it was clearly different from these two precursors.Isolation of l-dopa from the rice koji made by culture of Aspergillus and also from sake and sake cake was first carried out in the present work. |
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