Regulation of Nicotine Production by Auxins in Tobacco Cultured Cells in vitro |
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| Authors: | Masayuki Takahashi Yasuyuki Yamada |
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| Institution: | Department of Agricultural Chemistry, Kyoto University, Kyoto, 606, Japan |
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| Abstract: | A thermostable aminopeptidase, called aminopeptidase T, from the extract of Thermus aquaticus YT-1 was purified and characterized. The enzyme had a dimeric structure, its relative molecular mass being 108,000 by gel filtration, and 48,000 by SDS-PAGE. The optimum pH of the enzyme activity was in the range of 8.5 to 9.0. The enzyme was significantly thermostable as it still retained 60% of its original activity even after heat treatment for 20 hr at 80°C. The enzyme activity was inhibited by metal-chelating agents. The enzyme had a low substrate specificity. |
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