Enzymic Hydrolysis of Soybean Phosphatidyl Inositol by Trimeresurus flavoviridis Venom and a Pancreatic Lipase Preparation

We have made an investigation on the specific liberation of fatty acids from the molecules of soybean phosphatidyl inositol by Trimeresurus flavoviridis venom and a pancreatic lipase preparation in order to utilize the reaction for the study of the fatty acid distribution in phosphatidyl inositols. Both the venom and a pancreatic lipase preparation liberated about one half of the total fatty acids in phosphetidyl inositol, leaving probably a lysophosphatidyl inositol which contained mostly saturated fatty acids, whereas the most part of the component unsaturated acids was found in the liberated acids. The formation of the other hydrolysis products by these enzymes is also discussed.