Effect of Ca2+ Ions on the Viscometric Behavior of F-actin |
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Authors: | Ryo Nakamura |
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Affiliation: | Laboratory of Food Science and Technology (Animal Products), Faculty of Agriculture, Nagoya University, Nagoya |
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Abstract: | Changes in the viscosity of the F-actin solutions which occur on addition of Ca2+ ions were investigated. The viscosity of F-actin decreased on addition of Ca2+ ions. The amount of Ca2+ ions needed to decrease the viscosity changed with pH of the solution, namely, 20~30 mm at pH 7, 15~20 mm at pH 6 and 5~10mm at pH 5.5. Other divalent cations had the same action on F-actin, but monovalent cations did not affect the F-actin viscosity even at the concentration as high as 1 m. Intrinsic viscosity of F-actin with and without Ca2+ions was 250 ±40 (ml/g) and 670 ±80 (ml/g), respectively. The cause of this viscosity change was discussed from the results of electron microscopic observation and light scattering measurements. |
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Keywords: | Mir-655-3p PTTG1 non-small cell lung cancer migration invasion |
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