Production and Properties of Alkaline Dextranase from a Newly Isolated Brevibacterium |
| |
Authors: | Tsutomu Yamaguchi Shinobu Gocho |
| |
Institution: | Research Laboratory, Toyo Jozo Co., Ltd., Ohito-cho, Shizuoka-ken, Japan |
| |
Abstract: | About 500 strains of dextranase producing microorganisms were examined in detail for pH- activity and enzyme stability. A gram positive bacterium identified as belonging to the genus Brevibacterium was found to produce alkaline dextranase. Maximal dextranase synthesis was obtained when grown aerobically at 26°C for 3 days in a medium containing 1 % dextran, 2% ethanol, 1 % polypeptone and 0.05 % yeast extract together with trace amounts of inorganic salts.Brevibacterium dextranase had an optimum pH of 8.0 for activity at 37°C and an optimal temperature at 53°C at pH 7.5. The enzyme was quite stable over the range of pH 5.0 to 10.5 on 24 hr incubation at 37°C, especially on alkaline pH. The enzyme was also heat stable at 60°C for 10 min. |
| |
Keywords: | |
|
|