Production of Nucleic Acid Related Substances by Fermentative Processes |
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Authors: | Masanaru Misawa Takashi Nara Kiyoshi Udagawa Shigeo Abe Shukuo Kinoshita |
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Affiliation: | Tokyo Research Laboratory, Kyowa Hakko Kogyo Co., Ltd. |
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Abstract: | It was found that a guanine and adenine-requiring mutant of Micrococcus glutamicus accumulated the ultraviolet-absorbing substance in the culture fluid. A KY9978 strain, which accumulated the largest amount of the substance, we selected from the guanine auxotrophs derived from the guanineadenine doubleless mutant. The substance was isolated in a crystalline form from the culture fluid by the use of ion exchange resins, Diaion SA 21A and SK No. 1, and identified chemically and enzymatically as 5′-xanthylic acid, an intermediate from 5′-inosinic acid to 5′-guanylic acid on the purine nucleotide biosynthesis. |
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