Distribution and Properties of NAD Phosphorylating Reaction |
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Authors: | Masaaki Kuwahara Takashi Tachiki Tatsurokuro Tochikura Koichi Ogata |
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Affiliation: | 1. Department of Food Science, Kagawa University, Miki, Kagawa;2. Department of Food Science and Technology, Kyoto University, Kyoto;3. Department of Agricultural Chemistry, Kyoto University, Kyoto |
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Abstract: | Distribution of NAD phosphorylating reactions, phosphorylation through NAD kinase and phosphotransferase, was investigated. NAD kinase activity was distributed rather widely in bacteria, whereas phosphotransferase activity with p-NPP and NAD was limited to a few genera. Proteus mirabilis showed strong activity of phosphotransferase besides NAD kinase activity.Partial purification of the phosphotransferase was attempted. The enzyme preparation possessed phosphatase activity as well as phosphotransferase activity. Phosphorylation of NAD proceeded maximally under the conditions below pH 4.0. Cu2+ showed stimulating effect on the activity. Besides p-NPP and phenylphosphate, various nucleotides, especially 2′ (or 3′) isomers, served as excellent phosphoryl donors, and various kinds of nucleosides and nucleotides were phosphorylated to form nucleoside monophosphates and nucleoside diphosphates. |
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Keywords: | green tea polyphenols (–)-epigallocatechin gallate Porphyromonas gingivalis adherence |
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