Isolation of Alkaline Proteinase from Aspergillus sojae in Homogeneous Form |
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Authors: | Kazuya Hayashi Danji Fukushima Koya Mogi |
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Institution: | Central Research Institute of Kikkoman Shoyu Co., Ltd., Noda-shi, Chiba-ken |
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Abstract: | The alkaline proteinase of Aspergillus sojae was isolated in gram quantities as a homogeneous form. The purification procedures were, (1) batchwise-treatment with ion exchange resin Duolite CS 101, (2) fractional precipitation with ammonium sulfate, (3) precipitation with acetone, (4) column chromatography on DEAE-cellulose, and (5) gel filtration with Sephadex G-100. The recovery of the activity was about 12%. The purified enzyme preparation was found to be homogeneous by several criteria such as ultracentrifugation, paper and moving-boundary electrophoreses, etc. Any kinds of carbohydrate and phosphorus were not detected in this preparation, suggesting that this enzyme is a simple protein. |
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