Studies on the Induced Synthesis of Maleate cis-trans Isomerase by Malonate |
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Authors: | Yoshichika Takamura Tomiko Takamura Masami Soejima Teijiro Uemura |
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Affiliation: | 1. Department of Agricultural Chemistry, Faculty of Agriculture, Ibaraki University, Ibaraki;2. Institute of Applied Microbiology, The University of Tokyo, Tokyo |
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Abstract: | Maleate cis-trans isomerase in Alcaligenes faecalis IB–14 was induced by malonate and purified about 100-fold over the crude cell-free extract by treatments of ammonium sulfate fractionation, Sephadex G–100 gel filtration, DEAE-cellulose and DEAE-Sephadex A–50 column chromatography. The preparation was shown to be monodisperse on ultracentrifugal analysis and Svedberg value was found to be 3.84 S.The enzyme was most active at pH value around 8.3 and was stable over the range of pH 5.0 to 7.0 in the presence of dithiothreitol (DTT) for a few weeks, but in the absence of it, the enzyme activity was markedly decreased, especially in the alkaline region. The enzyme activity was inhibited by various sulfhydryl reagents and oxidizing agents, whereas it was not affected by metal chelating agents. The inhibition by Hg2+ and PCMB was overcome by the addition of sulfhydryl compounds such as DTT, 2-mercaptoethanol, l-cysteine and glutathione. It was observed that the enzyme did not require co-factor for its function.Kinetic studies showed that Michaelis constant for maleate was 2.8×10?3 m and the enzyme did not catalyze the reverse reaction. |
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Keywords: | premature antibody myoglobin acute myocardial infarction protein G rapid reaction kinetics |
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