Preliminary Investigation on Protein Bodies of Soybean Seeds

With the purpose of separation and isolation of protein bodies from soybean, soybean seeds were homogenized in oil and fractionated by successively adjusting densities of extracts with carbon tetrachloride. Isolated protein bodies consist of about 10% nitrogen, 0.8% phosphorus, 8.5% sugar, 7% ash and 0.5% RNA. Over 93% of protein in the bodies is found as particle-bound protein which is insoluble in 15% Carbowax 6000 solution but soluble in 10% sodium chloride solution. Protein bodies in intact cells, isolated bodies and those treated with Carbowax 6000 solution were respectively observed by electron-microscopy.