S-SI,a New Alkaline Protease Inhibitor from Streptomyces albogriseolus S-3253

This study investigated the production of ethanol from unutilized branches pruned from pear trees by steam explosion pretreatment. Steam pressures of 25, 35, and 45?atm were applied for 5?min, followed by enzymatic saccharification of the extracted residues with cellulase (Cellic CTec2). High glucose recoveries, of 93.3, 99.7, and 87.1%, of the total sugar derived from the cellulose were obtained from water- and methanol-extracted residues after steam explosion at 25, 35, and 45?atm, respectively. These values corresponded to 34.9, 34.3, and 27.1?g of glucose per 100?g of dry steam-exploded branches. Simultaneous saccharification and fermentation experiments were done on water-extracted residues and water- and methanol-extracted residues by Kluyveromyces marxianus NBRC 1777. An overall highest theoretical ethanol yield of 76% of the total sugar derived from cellulose was achieved when 100?g/L of water- and methanol-washed residues from 35?atm-exploded pear branches was used as substrate.