Effect of Cycloheximide on Development of Mitochondria in Germinating Pea Cotyledons |
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| Authors: | Yoshihiko Nawa Tadashi Asahi |
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| Institution: | Laboratory of Biochemistry, Faculty of Agriculture, Nagoya University, Bunkyo-ku, Chikusa, Nagoya, Japan |
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| Abstract: | Polyethylene glycol (PEG) mediated transfection of Lactobacillus casei ATCC 27092 protoplasts by phage PL-1 DNA was done. The protoplasts were obtained by treatment with purified PL-1 phage N-acetylmuramidase in the presence of citrate. Optimum conditions for transfection were 50% PEG 4,000, 15 µg protamine sulfate/ml, 0.15 m sucrose, and 10 m m MgSO4 in MR medium (pH 6.0). The extent of transfection was proportional to the amounts of DNA added, and the greatest efficiency of transfection after a 10-min incubation was about 3.3 × 105 PFU/µg DNA. The eclipse period of growth of progeny phages in the transfectants was 3 hr and the average burst size was 200. |
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| Keywords: | pyruvate-formate lyase formate dehydrogenase nitrate reductase denitrification Fusarium oxysporum |
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