Studies on the Autolysis of Aspergillus oryzae |
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| Authors: | Takeshi Uozumi Gakuzo Tamura Kei Arima |
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| Institution: | Department of Agricultural Chemistry, The University of Tokyo, Tokyo |
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| Abstract: | Properties of nuclease O, a new intracellular enzyme which was partially purified from autolyzate of Asp. Oryzae,1) are described in this paper. The purified enzyme preferentially depolymerized RNA and heat denatured DNA, but apparently did not attack native DNA. It was activated by 0.1 mm Mg2+ or Mn2+, and inactive in the presence of EDTA. Optimum pH of the activity were 7.7 for DNA and 8.2 for RNA. By heat treatment (60°C, 10 min at pH 6) the nuclease completely lost its activity for RNA and DNA. Optimum concentration of Tris buffer for enzymatic activity was 0.15~0.2m. |
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