Studies on the Autolysis of Aspergillus oryzae |
| |
Authors: | Tadayuki Hino Takeshi Uozumi Gakuzo Tamura Kei Arima |
| |
Institution: | Department of Agricultural Chemistry, The University of Tokyo, Tokyo |
| |
Abstract: | Mode of action of crystalline nuclease O obtained from autolyzed Aspergillus oryzae on RNA and synthetic homopolymers was examined. Crystalline nuclease O had no strict base specificity, although the velocity of hydrolysis was poly A > poly U > RNA > poly C. This enzyme did not degrade poly G. Digestion of high molecular weight RNA with an excess of this enzyme produced mono-, di- and trinucleotides with 5′-terminal phosphate. The amount of mono-, di- and trinucleotides was, respectively, 13.6, 70.0 and 16.4% of total degradation products. All the four bases were detected in mononucleotide fraction and 3′-terminals and 5′-terminals of oligonucleotides. |
| |
Keywords: | dehydrodolichyl diphosphate synthase dolichol Z Z E-geranylgeranyl diphosphate microsomes trypsin |
|
|