Preparation and Properties of Crystalline Glucoamylase from Endomyces Species IFO 0111

Glucoamylase [EC 3. 2. 1. 3] of Endomyces sp. IFO 0111 was purified from the culture filtrate by chromatography on CM-cellulose, and isolated in crystalline state. The crystalline enzyme was free from α-amylase, and displayed apparent homogeneity upon ultracentrifugation and electrophoresis. The molecular weight was 55,000 by the Archibald’s method. The results of amino acid analysis revealed the absence of methionine residue in this enzyme protein. The purified enzyme acted on starch hydrolyzing up to about 80%, whereas the crude enzyme hydrolyzed the same substrate almost completely. Some other properties were also included in this paper.