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Gibberellins in Immature Seeds of Canavalia
Authors:Noboru Murofushi  Nobutaka Takahashi  Takao Yokota  Jiro Kato  Yoshitaka Shiotani  Saburo Tamura
Affiliation:1. Department of Agricultural Chemistry, The University of Tokyo, Tokyo;2. Department of Biology, University of Osaka Prefecture, Sakai, Osaka
Abstract:Two novel gibberellins, GA21 (I) and GA22 (II), were isolated from immature seeds of sword bean, Canavalia gladiata DC. The isolation procedure of these substances as well as their growth-promoting effects on dwarf maize mutants d1 and d5, rice, cucumber and dwarf peas (Progress No. 9) are described.

The structures of two new gibberellins, GA21 and GA22, isolated from immature seeds of sword bean, were determined as 4aα, 7α-dihydroxy-8-methylenegibbane-1, 1, 10β-tricarboxylic acid 1→4a lactone (II) and 4aα, 7α-dihydroxy-l β-hydroxymethyl-8-methylenegibb-2-ene-1α, 10β-dicaboxylic acid 1→4a lactone (IV), respectively, on the basis of chemical and physicochemical studies.
Keywords:α-galactosidase  Mortierella vinacea  cDNA cloning  galactomanno-oligosaccharides  specificity of α-galactosidase
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