Micro-analysis of Amino Acids with Fluorescein-isothiocyanate

The separation and identification of fluorescein-thiocarbarnyl (FTC-) amino acid II were accomplished by one- and two-dimensional thin-layer chromatography. The authentic samples for identification of amino acids were synthesized with fluorescein-isothiocyanate II (FITC II) and 21 amino acids. These FTC-amino acids were studied spectrometrically.

For quantitative estimation of FTC-amino acids, the fluoroscopy was used. It was found that the fluorescence intensity was proportional to the concentration of FTC-amino acids in 2 pmole/ml to 20 nmole/ml range. Recovery of FTC-derivatives on silica gel plate was about 80%.