2-Keto-l-gulonic Acid Fermentation |
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Authors: | Masao Isono Itaru Nakanishi Ken-ichi Sasajima Kazuwo Motizuki Toshihiko Kanzaki Hisayoshi Okazaki |
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Institution: | Microbiological Research Laboratories, Research and Development Division, Takeda Chemical industries, Ltd., Osaka, Japan |
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Abstract: | A number of bacterial strains from type culture collections and natural sources were examined in their metabolic characteristics toward sorbitol and l-sorbose.Paper chromatographic analyses of sorbitol and l-sorbose metabolites obtained from the cultures of various bacteria revealed that the organisms producing 2-keto-l-gulonic acid from sorbitol were merely found in the genera Acetobacter, Gluconobacter and Pseudomonas, whereas those producing the acid from l-sorbose were distributed in the twelve genera of bacteria: Acetobacter, Alcaligenes, Aerobacter, Azotobacter, Bacillus, Escherichia, Gluconobacter, Klebsiella, Micrococcus, Pseudomonas, Serratia and Xanthomonas.G. melanogenus, which was characterized by excellent production of 2-keto-l-gulonic acid from sorbitol, also formed several other sugars and sugar acids as the sorbitol metabolites. These compounds were identified to be d-fructose, l-sorbose, d-mannonic acid, L-idonic acid, 2-keto-d-gluconic acid and 5-keto-d-mannonic acid, respectively, by means of two-dimensional paper chromatography.Bacteria producing 2-keto-l-gulonic acid from sorbitol were usually isolated from fruits but not from soil. |
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