Synthesis of l,7-Diphenyl-l,3-heptadien-5-one,One of the Components in the Fresh Catkin of Alnus pendula |
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Authors: | Masayuki Sakakibara Kenji Mori Masanao Matsui |
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Affiliation: | Department of Agricultural Chemistry, The University of Tokyo, Tokyo |
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Abstract: | Several microorganisms capable of utilizing 1-aminocyclopropane-1-carboxylate (ACPC) were isolated from soil. A bacterium which belongs to Pseudomonas accumulated cellular α-aminobutyrate with consumption of ACPC and cells incubated with ACPC medium had the activity deaminating the substrate to form α-ketobutyrate. An enzyme, ACPC deaminase, was highly purified and its molecular weight, substrate specificity and absorption spectrum were investigated. These results suggested that this enzyme was a pyridoxal 5′-phosphate enzyme which has the molecular weight of 104000 and high specificity for ACPC, Km= 1.5 mM. A yeast, Hansenula saturnus, is also capable of forming ACPC deaminase, which has a lower molecular weight, 69000, and higher Km value, 2.6 mM. |
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