Utilization of Hydrocarbons by Microorganisms |
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Authors: | Yukio Imada Joji Takahashi Kōichi Yamada Kinya Uchida Kō Aida |
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Institution: | 1. Department of Agricultural Chemistry, The University of Tokyo, Tokyo;2. Institute of Applied Microbiology, The University of Tokyo, Tokyo |
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Abstract: | As already reported, strain S1OB1 was found to accumulate l-glutamic acid in a thiamine-deficient medium at the sole expense of hydrocarbon. In order to elucidate the biosynthetic pathway of l-glutamic acid, first of all, the incorporation of molecular oxygen into l-glutamic acid was examined. l-Glutamic acid accumulated under 18O-enriched atmosphere was separated, purified, identified and found to have been enriched with 18O. This results indicate the occurrence of oxygenase reaction involving addition of molecular oxygen. From a postulated biosynthetic pathway of l-glutamic acid, theoretical 18O content was calculated and compared with experimental one. 18O content of cells grown on n-alkane or glucose was also examined. |
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