The Influence of Measurement Parameters on the Specific Rotation of Amino Acids |
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Authors: | Koichi Hayashi Yutaka Fujii Ryuichi Saito Hiroyuki Kanao Tetsuo Hino |
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Affiliation: | Kawasaki Factory, Ajinomoto Co., Inc., Kawasaki, Japan |
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Abstract: | The effects of solute and hydrochloric acid concentrations on optical rotation were studied using 20 naturally occuring amino acids.There appeared to be no common factor among the amino acids as far as the inclination of optical rotation was concerned. Lutz-Jirgenson’s rule could be applied to few amino acids in the cationic form. Therefore, in the determination of the optical rotation, the concentration of the solute, nature of solvent and temperature must be rigorously controlled. The optical conditions of measurement and the specific rotation of 20 amino acids were recommended based on this work. |
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