Studies on Production of Nucleic Acid and its Related Compounds by Microorganisms

Adenosine was produced from adenine by means of fermentation using Bacillus subtilis Marburg 160-88 (st^r, try^-, pur^-). For the study on adenosine production, experiments concerning with pre-culture age, inoculum size, fermentation period, concentration of adenine, carbon source, nitrogen source and supplement of vitamins were carried out by test tube shaker. Furthermore the time course of fermentation was observed using jar fermentor. And it was proved that adenosine was produced about 1 mg/ml during the first 40 hrs of fermentation in the glucose mineral medium containing 1~2 mg/ml of adenine. This fermentation procedure seems to be one of economical methods for adenosine production.