Isolation and Characterization of Antitumor Lipopolysaccharide from Proteus mirabilis |
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| Authors: | Mamoru Arai Masaki Nakahara v Hamano Hiroshi Okazaki |
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| Affiliation: | Fermentation Research Laboratories, Sankyo Co., Ltd., No. 2–58, Hiromachi 1-chome, Shinagawa-ku, Tokyo 140, Japan |
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| Abstract: | Systematic isolation of the cell constituents of Proteus mirabilis RMS–203 was performed to find out localization of antitumor principle only in the lipopolysaccharide (LPS) layer of the cell wall fraction.LPS with strong antitumor activity was extracted from P. mirabilis RMS–203 by phenol-water method followed by purification on DEAE-Sephadex A–50 column chromatography.The main components of purified LPS were galactose, hexosamine, 2-keto-deoxy-octonic acid (KDO), myristic acid, β-hydroxymyristic acid and α,ε-diaminopimelic acid.The minimal effective dose of LPS against Ehrlich solid carcinoma in mice was 0.1~1.0 μg/mouse. LD50 in mice and pyrogenicity in rabbits were 28 mg/kg and 10?3–10?5 μg/rabbit, respectively. |
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| Keywords: | aphidicolin diterpene total biosynthesis heterologous expression Aspergillus oryzae |
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