The State of Amino Acid Residues in Alkaline Protease Produced by Bacillus No. 221 |
| |
| Authors: | Keiji Nakamura Ayako Matsushima Koki Horikoshi |
| |
| Institution: | 1. The Institute of Physical and Chemical Research, Wako-shi, Saitama-ken, 351;2. Tokyo Institute of Technology, Ohokayama, Meguro-ku, Tokyo |
| |
| Abstract: | The state of amino acid residues in alkaline protease of Bacillus No. 221 and that of subtiiisin BPN’ were compared by spectrophotometric tiiration of tyrosine residues and by several reagents: β-naphtoqumone-4,6-disulfonic acid and monochlorofluoroquinone for amino groups, H2O2-dioxane for tryptophan, glyoxal for arginine, and tetranitromethane for tyrosine.The reactivity of both proteases was fairly similar to those reagents.The helix content of alkaline protease of Bacillus No. 221 (37%) was higher than that of subtilisin BPN’ (20%).The Km and Vmax of alkaline protease of Bacillus No. 221 toward ATEE and BTEE were obtained from Lineweaver-Burk plot and compared with those of α-chymotrypsin and subtiiisin BPN’. |
| |
| Keywords: | prostate glycosphingoplipid fucosylation lectin blot |
|
|
