Interconversion of Two Lipases from Rhizopus delemar

Rhizopus (Rh.) delemar were cultivated under the various conditions and the productivity of three lipases (A, B, and C) were examined. There seemed to be no remarkable change of A-lipase production so far as examined, however, the productions of B- and C-lipases were changed with correlation depending on the composition of the medium. B-lipase was increasingly produced as much as C-lipase production decreased by the presence of phospholipid in a culture medium. The property of C-lipase was so changed by the incubation with phospholipid as to agree with that of B-lipase. Besides the above, the property of B-lipase was changed by its purification to that of C-lipase.

From these results, it seems that B-lipase protein is the same as that of C-lipase and a phospholipid is related to the intercoversion from C-lipase to B-lipase.