Microbial Production of l-Glutamic Acid by Glycerol Auxotrophs |
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Authors: | Yoshio Nakao Masakazu Kikuchi Masaru Suzuki Muneharu Doi |
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Affiliation: | Microbiological Research Laboratories, Research and Development Division, Takeda Chemical Industries, Ltd., Osaka, Japan |
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Abstract: | To establish a novel process for the production of l-glutamic acid from n-paraffins, a glycerol auxotroph GL-21, a new type mutant, was successfully obtained from Corynebacterium alkanolyticum No. 314 by treatment with N-methyl-N′-nitro-N-nitrosoguanidine. This auxotroph required glycerol for its growth regardless of the carbon source used.At 72 hr, this mutant GL-21 produced about 40 mg/ml of l-glutamic acid from n-paraffins in the culture broth at 0.01 per cent addition of glycerol in the absence of penicillin.A thiamine auxotroph, a biotin auxotroph and an oleic acid auxotroph were also obtained by a similar technique, but these auxotrophs were found to be inapplicable for the production of l-glutamic acid from n-paraffins. |
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Keywords: | biofilm formation Caenorhabditis elegans κ-casein macropeptide Listeria monocytogenes two-dimensional gel electrophoresis |
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