Isolation and Properties of Acid Phosphatases of Sweet Potato Roots |
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| Authors: | Tadashi Asahi Katsuhiro Sakano Takeo Nomura Hiroshi Ogawa Ikuzo Uritani |
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| Institution: | Laboratory of Biochemistry, Faculty of Agriculture, Nagoya University, Nagoya |
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| Abstract: | Acid phosphatase of sweet potato root tissue was found to consist of five components by diethylaminoethyl-cellulose column chromatography, and each component was isolated by the rechromatography. They were not separated by Sephadex G-200 gel filtration. All components hydrolyzed various phosphate compounds including phosphomonoester- and pyrophosphate-linkages. Their optimum pH values were in the range of pH 5 to 6. However, there were observed some differences in optimum pH, Michaelis constant for various substrates and relative maximum velocity among the components. |
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