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2-Ketogluconate Reductase Inhibition by Oxalate |
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| Authors: | Minoru Ameyama Toshikazu Chiyonobu Osao Adachi |
| Affiliation: | Department of Agricultural Chemistry, Yamaguchi University, Yamaguchi |
| Abstract: | The active site of α-glucosidase from Mucor javanicus IFO 4570 was investigated by kinetic studies. Competition between maltose and soluble starch, and linearity of Lineweaver-Burk plots for the mixed substrates were observed. The dependence of the apparent maximum velocities agreed with those predicted for a single active site mechanism. These results suggest that the enzyme hydrolyzes maltose and soluble starch at a single active site. |
| Keywords: | defatted rice bran subcritical condition acetone 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity antioxidative activity |