Purification of Mucor Lipases and Their Properties |
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| Authors: | Kozo Nagaoka Yujiro Yamada |
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| Institution: | Meiji Seiko Kaisha, Ltd., Research Laboratories, Morooka-cho, Kohoku-ku, Yokohama |
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| Abstract: | Olive oil hydrolyzing fraction (F-3) of Mucor lipase was separated into two fractions, F-3A and F-3B, by means of CM-Sephadex column chromatography. Both fractions were homogeneous and F-3A was crystallized. The pH optimum for olive oil hydrolysis of F-3A was at 9.0 and that of F-3B was at 8.0.Chain specificities of the both enzymes were different with each other. Sedimentation coefficient (S20, w) of F-3A was 2.8 S and that of F-3B was 3.1 S.Molecular weights calculated from sedimentation equilibrium data were 25,400 for F-3A and 29,000 for F-3B. |
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