Polarographic Behavior of Thiolated Natural and Synthetic Polymers

Polarographic protein waves were studied by using model samples. Two samples were prepared by thiolation of natural and synthetic polymers which show no catalytic wave in the ammoniacal cobalt buffer. The one was made from bacterial α-amylase by thiolation with N-acetylhomocysteine and the other was made from polyvinylalcohol by esterification with thioglycolic acid. These thiolated polymers showed typical double waves similar to protein waves both in cobaltous and cobaltic media though minute differences were present between the waves of thiolated polyvinylalcohol and those of proteins.