Inactivation of Bacteriophages by Thiol Reducing Agents |
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Authors: | Akira Murata Kazuko Kitagawa Hiromi Inmaru Rinjiro Saruno |
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Affiliation: | Department of Agricultural Chemistry Saga University, Laboratory of Applied Microbiology, Saga, Japan, 840 |
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Abstract: | Adaptation of the vaccinia virus expression system to HeLa S3 suspension bioreactor culture for the production of recombinant protein was conducted. Evaluation of hollow fiber perfusion of suspension culture demonstrated its potential for increased cell density prior to infection. The hollow fiber was also used for medium manipulations prior to infection. Two process parameters, multiplicity of infection (MOI) and temperature during the protein production phase, were evaluated to determine their effect on expression of the reporter protein, enhanced green fluorescent protein (EGFP). An MOI of 1.0 was sufficient for infection and led to the highest level of intracellular EGFP expression. Reducing the temperature to 34 °C during the protein production phase increased production of the protein two-fold compared to 37 °C in spinner flask culture. Scaling up the process to a 1.5-liter bioreactor with hollow fiber perfusion led to an overall production level of 9.9 μg EGFP/106 infected cells, or 27 mg EGFP per liter. |
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Keywords: | vaccinia enhanced green fluorescent protein (EGFP) perfusion hollow fiber HeLa S3 |
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