Differential Effects of Nerve Growth Factor and Ciliary Neuronotrophic Factor on Catecholamine Storage and Catecholamine Synthesizing Enzymes of Cultured Rat Chromaffin Cells |
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| Authors: | K. Seidl M. Manthorpe S. Varon K. Unsicker |
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| Affiliation: | Department of Anatomy and Cell Biology, University of Marburg, F.R.G.;Department of Biology, School of Medicine, University of California at San Diego, La Jolla, California, U.S.A. |
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| Abstract: | The effects of nerve growth factor (NGF) and ciliary neuronotrophic factor (CNTF) on catecholamine content and in vitro activities of tyrosine hydroxylase (TH) and phenylethanolamine N-methyltransferase (PNMT) were studied in adrenal chromaffin cells cultured from 8-day-old rats. Both NGF and CNTF enhanced chromaffin cell survival and partially prevented losses of adrenaline during the 4-day culture period in a dose-dependent manner. CNTF was more potent, although cellular levels of adrenaline and noradrenaline were not maintained. NGF did not add to the effect of CNTF. The effect of CNTF on catecholamine storage was not accompanied by changes in the activities of TH and PNMT. In contrast, NGF induced TH but not PNMT activity. These data indicate differences between the mechanisms by which NGF and CNTF affect adrenal chromaffin cells. |
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| Keywords: | Nerve growth factor Ciliary neuronotrophic factor Chromaffin cells Culture Catecholamines Enzymes |
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