Metabolism of triacylglycerol in developing rat brain |
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Authors: | Harold W Cook |
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Institution: | (1) Departments of Pediatrics and Biochemistry Atlantic Research Centre for Mental Retardation, Dalhousie University, 5849 University Avenue, B3H 4H7 Halifax, Nova Scotia |
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Abstract: | Metabolism of triacylglycerol (TAG) in developing brain has been examined. TAG is a relatively minor fraction of brain lipid in both suckling and adult rats and cannot be accounted for as entrapped blood. When glycerol tri1-14C]oleate and 2-3H]glycerol trioleate were simultaneously injected intracerebrally into suckling rats, both labels appeared in diacylglycerol and the major phospholipids; acyl chain label was incorporated more extensively at early time points, with choline phosphoglycerides being most actively labeled. With 1-14C]fatty acids and 2-3H] glycerol administration, the specific activity of TAG was much greater than that of the more abundant phospholipids. Although direct acyl exchange between TAG and phospholipids was not demonstrated, relationships of TAG to selective mechanisms of phosphoglyceride synthesis were indicated.Abbreviations used TAG
triacylglycerol
- DAG
diacylcerol
- HPLC
high performance liquid chromatography
- CoA
coenzyme A
- BSA
bovine serum albumin
- TLC
thin layer chromatography
- DPM
disintegrations per minute
- ATP
adenosine triphosphate
- GLC
gas liquid chromatography
- PC
choline, phosphoglyceride
- PE
ethanolamine phosphoglyceride
- PS
serine phosphoglyceride
- PI
inositol phosphoglyceride |
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