Stress-induced peptide release from rat intermediate pituitary |
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Authors: | James A Carr Linda C Saland Ann Samora Sima Desai Salvador Benevidez |
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Institution: | (1) Department of Anatomy, School of Medicine, University of New Mexico, 87131 Albuquerque, NM, USA |
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Abstract: | Summary We tested the hypothesis that acute restraint stress results in ultrastructural evidence for enhanced release of alpha-melanocyte-stimulating hormone (-MSH) and -endorphin from the intermediate lobe (IL) of the rat pituitary. Measurements of plasma -MSH-and -endorphin-immunoreactivity (ir) were used to confirm ultrastructural findings. Plasma -MSH-ir was elevated after 20 and 30 min of restraint while plasma -endorphin-ir peaked 10 min after the onset of restraint. Ultrastructural analysis revealed a decrease in the content of secretory granules within IL cells of stressed rats. Analysis of Golgi-related immature secretory granules in IL cells indicated that new peptide synthesis was not enhanced after 30 min of restraint. These results confirm previous studies showing and elevation of plasma -endorphin and -MSH-ir during acute restraint. Furthermore, these results indicate that quantitative analysis at the ultrastructural level can be used to assess peptide release from IL secretory cells during stress. |
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Keywords: | Pituitary gland pars intermedia Stress Secretory granules -MSH" target="_blank">gif" alt="agr" align="BASELINE" BORDER="0">-MSH -Endorphin" target="_blank">gif" alt="beta" align="MIDDLE" BORDER="0">-Endorphin Rat (Sprague-Dawley) |
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