Production of toluene cis-glycol by a recombinant Escherichia coli strain in a two-liquid phase culture system |
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Authors: | J. T. Tsai L. P. Wahbi G. A. Dervakos G. M. Stephens |
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Affiliation: | (1) Department of Chemical Engineering, UMIST, PO Box 88, M60 1QD Manchester, UK |
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Abstract: | Summary The recombinant Escherichia coli strain, TG2 (p1/1), can be used for biotransformation of toluene to toluene cis-glycol. However, toluene caused substantial inhibition of both growth and toluene cis-glycol production at concentrations above 0.04%. Tetradecane was found to be non-toxic at a phase ratio of 0.23 and provided protection against toluene toxicity. Thus, growth was still possible in the presence of 1.5% toluene. Furthermore, the yield of toluene cis-glycol was increased 1.7-fold compared with the yield in the absence of tetradecane. |
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