Utility of barley and wheat simple sequence repeat (SSR) markers for genetic analysis of Hordeum chilense and tritordeum |
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Authors: | Hernández P Laurie D A Martín A Snape J W |
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Institution: | (1) Instituto de Agricultura Sostenible (CSIC), Apartado 4084, 14080 Cordoba, Spain e-mail: ge1hemop@uco.es Tel.: +34-957-499210, Fax: +34-957-499252, ES;(2) John Innes Centre, Norwich Research Park, Colney, Norwich NR4 7UH, UK, GB |
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Abstract: | A selection of 36 wheat and 35 barley simple sequence repeat markers (SSRs) were studied for their utility in Hordeum chilense. Nineteen wheat and nineteen barley primer pairs amplified consistent H. chilense products. Nine wheat and two barley SSRs were polymorphic in a H. chilense mapping population, producing codominant markers that mapped to the expected homoeologous linkage groups in all but one case.
Thirteen wheat and 10 barley primer pairs were suitable for studying the introgression of H. chilense into wheat because they amplified H. chilense products of distinct size. Analysis of wheat/H. chilense addition lines showed that the H. chilense products derived from the expected homoeologous linkage groups. The results showed that wheat and barley SSRs provide a valuable
resource for the genetic characterization of H. chilense, tritordeums and derived introgression lines.
Received: 20 November 2000 / Accepted: 12 April 2001 |
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Keywords: | Hordeum chilense Tritordeum Introgression Marker-assisted selection Mapping Microsatellite SSR |
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