A short procedure to measure phospholipid exchange in mitochondria and microsomes |
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| Authors: | S A Kamath E Rubin |
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| Affiliation: | Department of Pathology, Mount Sinai School of Medicine, New York, New York 10029 USA |
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| Abstract: | A short procedure is described to study the exchange of phospholipids between rat liver organelles in vitro. 32P-Labeled microsomes are bound to Ca2+, sedimented at 30g, and incubated with unlabeled post-700g-supernatant of liver homogenate. After recovering the originally labeled microsomes at 700g, mitochondria and microsomes of the unlabeled fraction are isolated and specific activity of 32P measured. Net transfer of phospholipids is comparable to that found after incubation of separate fractions. |
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