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Effects of auxin and light treatments of donor plants on shoot production fromindica-type rice (Oryza sativa L.) |
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| Authors: | V L M Pádua L D Fernandes D E de Oliveira E Mansur |
| Institution: | 1. University Federal do Rio de Janeiro, Instituto de Biologia, LGMV, Rio de Janeiro, RJ, Brazil 2. Universidade Federal do Rio de Janeiro, Instituto de Biologia, Departamento de Genetica, LGMV, Rio de Janeiro, RJ, Brazil 3. Universidade Federal do Rio de Janeiro, Horto Botanico do Museu Nacional, Rio de Janeiro, RJ, Brazil 4. Universidade do Estado do Rio de Janeiro, IBRAG, Labmit, Rua S?o Francisco Xavier 524, PHLC/sala 505, Rio de Janeiro, RJ, Brazil 5. Universidade do Estado do Rio de Janeiro, Instituto de Biologia, LABMIT/DBCG, Rua S?o Francisco Xavier 524-PHLC/sala 505, Rio de Janeiro, RJ, Brazil |
| Abstract: | An efficient clonal propagation procedure for a Brazilianindica rice subspecies was developed with shoot apex explants. Shoot apices were excised from 4-d-old seedlings and cultured on MS medium supplemented with 8.9 μM 6-benzyladenine. The efficiency of shoot production was influenced by growth regulators and light treatments to the donor plant. Explants derived from seedlings growth in the presence of 10.7 μM naphthaleneacetic acid and in the absence of light showed significantly increased regeneration capacity as compared to control explants. Anatomical analysis of the new shoot meristems revealed that they originated from preexisting apical and axillary meristem as well as from the mesocotyl parenchyma. |
| Keywords: | micropropagation shoot apex rice donor plant |
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