稳定同位素化学标记结合质谱技术在定量蛋白质组学中的应用

传统的蛋白质组定量策略主要是通过双向凝胶电泳来进行相对定量。由于该方法不能对相对分子质量极高或极低、等电点极酸或极碱和含量低的蛋白质以及膜蛋白质等进行有效分离和检测,所以已不能适应目前蛋白质组研究深入发展的需要。近年来,定量蛋白质组学的发展主要是以同位素亲和标签试剂为代表的、以质谱检测为核心的稳定同位素化学标记方法。稳定同位素化学标记结合质谱技术,使定量蛋白质组的分析更趋简单、准确和快速,具有良好的发展前景。本文对稳定同位素化学标记结合质谱技术在定量蛋白质组学中的研究进展进行了评述。

Application of Stable Isotopic Chemical Labeling Connected with Mass Spectrometry in Quantitative Proteomics

Two dimensional gel electrophoresis,traditionally used for relative quantitation,couldn't meet the need for further development of proteomics for being incapable of separating membrane proteins and proteins with extra high or low molecular weight,extra acid or base pI,and proteins with low concentration.A new gel-independent technology,stable isotopic labeling,emerged and made quantitation easier,more accurate and rapid.It has developed so quickly in recent years that it takes on behalf of development of current proteomics analysis technologies.In the review,the author stated the application of stable isotopic labeling in quantitative proteomics in the following aspects,such as stable isotope dilution technique and stable isotope chemical labeling.