A high-density differential screening strategy for identification of fovea genes with altered expression in the retina during aging |
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| Authors: | Richard Idem Somiari Paul Wong Steve Lance Bernstein |
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| Affiliation: | (1) Laboratory of Molecular Research, Department of Ophthalmology, University of Maryland, Baltimore, 10 South Pine street, MSTF 5-65, MD 21201, USA;(2) Department of Biological Sciences, University of Alberta, Edmonton, T6G 2E9, Canada |
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| Abstract: | A human fovea cDNA library was arrayed at high-density and reacted with age-specific (4 yr & 80 yr) retinal probes to identify genes with altered expression during aging. Using this approach, we screened 55,368 genes by Southern analysis and determined that  0.7% are differentially expressed in young and old tissues. Northern analysis of total RNA from retina of humans aged 2–94 yr show that expression of one of the clones identified (clone dd112) decreases with aging. |
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| Keywords: | aging fovea array retinal genes |
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