Cryopreservation of the edible alkalophilic cyanobacterium Arthrospira platensis |
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Authors: | Hideaki Shiraishi |
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Affiliation: | Division of Integrated Life Science, Graduate School of Biostudies, Kyoto University, Kyoto, Japan |
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Abstract: | Efficient cryopreservation conditions for the edible alkalophilic cyanobacterium Arthrospira (Spirulina) platensis were investigated using a model strain A. platensis NIES-39. As a result, it was found that more than 60% of cells were viable upon thawing, when they had been frozen at a cooling rate of approximately ?1 °C min?1 in the presence of 10% (v/v) dimethyl sulfoxide. Further examination with other Arthrospira strains showed that many of them had strain-dependent optimal conditions for cryopreservation. For example, the best freezing conditions for A. platensis SAG 21.99 were snap-freezing in liquid nitrogen in the presence of 5% (v/v) dimethyl sulfoxide, while they were slow cooling at approximately ?1 °C min?1 in the presence of 10% (v/v) methanol for A. platensis NIES-46, NIES-2308 and UTEX 1926. The variety of successful cryopreservation conditions presented in this study is useful when attempting to cryopreserve various Arthrospira strains. |
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Keywords: | cyanobacteria dietary supplements spirulina Arthrospira platensis cryopreservation |
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