Enhancing cellulase production by overexpression of xylanase regulator protein gene,xlnR, in Talaromyces cellulolyticus cellulase hyperproducing mutant strain |
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| Authors: | Naoyuki Okuda Hiroyuki Inoue Kazuhiko Ishikawa Tamotsu Hoshino |
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| Affiliation: | 1. Sugar &2. Bio Technology Department, Tsukishima Kikai Co Ltd, Chuo-ku, Tokyo, Japan;3. Research Institute for Sustainable Chemistry, National Institute of Advanced Industrial Science and Technology (AIST), Higashi-Hiroshima, Hiroshima, Japan;4. Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Ikeda, Osaka, Japan |
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| Abstract: | We obtained strains with the xylanase regulator gene, xlnR, overexpressed (HXlnR) and disrupted (DXlnR) derived from Talaromyces cellulolyticus strain C-1, which is a cellulase hyperproducing mutant. Filter paper degrading enzyme activity and cellobiohydrolase I gene expression was the highest in HXlnR, followed by C-1 and DXlnR. These results indicate that the enhancement of cellulase productivity was succeeded by xlnR overexpression. |
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| Keywords: | biomass cellulase xlnR Talaromyces cellulolyticus |
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